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| Frozen Tissue Grinding |
| CRYOPIX®
FROM ALPHELYS: FROZEN SAMPLES GRINDING |
1 SECOND OF GRINDING AT -140°C FOR AN UNEQUALED
PRESERVATION OF THE MOLECULAR CONTENT OF YOUR SPECIMEN AND
A HIGH EXTRACTION YIELD
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The grinding is the
prerequesite to many molecular extarctions to recover
DNAs, RNAs, proteins or other molecules.
Experience shows that grinding also constitutes the
most critical step at the origin of many alterations
and degradations of the molecules of interest as well
as important losses.
Various techniques are currently used every day to grind
frozen samples such as blade miller, bead grinder, hammer
miller of even hand used mortar and pillar.
Unfortunately, none of these techniques have demonstrated
a true efficiency for the extraction of molecules with
sufficiently high yield and quality.
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Loss of quality and yield due to
specimen heat up
Grinding heats up the specimen particularly due to frictions
but also due to some of the grinding techniques that cannot
grind frozen samples. In these conditions, degrading enzymes
are active and alter or even destroy the molecules of
interest. The use of buffers containing anti-enzyme reagent
are not sufficient. Indeed, as long as last the grinding,
not all of the specimen is in intimate contact with the
stabilizing reagent thus allowing for the enzymes to degrade,
and the more than the grinding procedure heats up the
specimen. Loss of quality, functionality and quantity
are observed. |
Low recovery
yield
Grinding is an important source of loss also from the
configuration of the grinding accessories. Recovering
powder from grinding can reveal very difficult and long
from blade miller or bead grinder where lots of biological
material can be lost. This is of course particularly critical
with human specimen where we can observe that samplings
are smaller and smaller due to earlier and earlier diagnostic. |
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CRYOPIX®
revolutionize grinding of frozen samples of small size
for molecular biology
ALPHELYS now proposes the le CRYOPIX®,
a new technology of grinding that permits to overcome
the two main limitations of regular grinding techniques
and simplifies this usually tedious and time consuming
procedure.
Here beside is an overview of the installation of an
CRYOPIX®
with the CRYOPIX®
itself on the left of the table, the control box of
the CRYOPIX®
on the right and on the floor at the left is the DEWAR
to inject liquid nitrogen vapours into the grinding
recipient of the CRYOPIX®.
In the incrusted picture is a technician is operation
on the CRYOPIX®.
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Maximize recovery yeilds
The grinding technology of the CRYOPIX®
is specially designed to reduce to the minimum
the losses of powder after grinding into grinding
accessories.
The grinding of the CRYOPIX®
occurs into a small round conical bottom polished
316L stainless steel recipient. Powder cannot
loose into corners or small cracks of the surface
that is perfectly polished and even. Acces to
the powder into this recipient is easy and the
powder can be readily recovered with a spatula
by user. Thanks to the profile of the recipient,
specimen as small as 50 mg and up to 1 g can be
grinded without threatening for powder losses.
Moreover, the grinder occurring at very low temperature,
degradation enzymes activities are inhibited.
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Here above is a
top view on the grinding recipient with a fragment
of a tumour (pink). Here beside on a blue background
is a detailed view of the grinding recipient with
the conical bottom. Here below is a view of a technician
recovering the powder after grinding of a breast
tumour with a spatula.
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Guarantee
a high quality for your molecules of interest
The CRYOPIX®
préserves the quality and functionality of
the molecules of interest by maintaining the specimen
at -140°C during the complete grinding procedure
with injection of liquid nitrogen vapours.
-140°C is precisely just below the last cristallisation
point of water. Moreover, at this very low temperature,
any enzymatic activities are very slow or completely
inhibited eliminating any risks of alteration or
degradation. You can thus recover your molecules
in the native state, without proteins denaturation
or lysis of your DNAs and RNAs.
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The injection
of liquid nitrogen vapours into the grinding recipient
maintains all parts in direct contact with the specimen
at very low temperature.
All molecular techniques tested on the molecules
extracted from grinded specimen have shown highly
reproducible and high lquality levels. Techniques
such as ELISA, immuoassays, mass spectrometry for
proteins, RT PCR, DNA and RNA chip have been applied
with success on CRYOPIX®
extracts.
The CRYOPIX®
is routinely used for the extraction of proteins,
DNAs and RNAs for the establishment of clinical
diagnosis.
Trials realized by different research teams have
shown excellent yields and quality on HP Agilent
profiles. Results have also shown excellent correlation
between DNAs and RNA/Protein expression levels.
RNAs are preserved regardless to their size.
The picture here above shows the grinding system
with liquid nitrogen vapours injection to maintain
very low temperature.
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| Grind multiple samples in a few
moment ! |
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The CRYOPIX®
grinds almost instantaneously any frozen specimen.
Just place the specimen, press the button, it is
grinded ! Grinding does not take more that the time
necessary to count a second ! You will never find
elsewhere a grinding procedure as fast. |
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Do not
threat any more the tediousness of grinding sessions,
it has become really simple now with CRYOPIX®
!
The CRYOPIX®
is a simple tool. The temperature set only requires 20
minutes and the device is ready for grinding. Tare your
tubes to receive the powder, place your specimen into
the recipient, press on the button to grind, collect the
powder and distribute lysis buffer. It is has simple to
do than to realize !
Very few parts are contaminated during grinding. Very
few time to spend in cleaning !
The liquid nitrogen is directly handled by the CRYOPIX®
avoiding all risks of burns for the user. Due to the manual
handling.
Moreover, the grinding requires the simultaneous application
on two buttons to occur thus obliging the user to maintain
his hands away from the moving parts of the CRYOPIX®
for a much safer manipulation. |
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Technical specifications
Grinding temperature : -135 à -140°C
Parts in contact with specimen: 316L polished stainless
steel. Cleaning with water and/or diluted bleach.
Power supply: 100-250 V, 50-60 Hz, 4A
Gas supply: dry gas nitrogen to pressurize liquid nitrogen
DEWAR.
Approx. liquid nitrogen consumption:
Approx. dry nitrogen gas consumption:
Weight CRYOPIX®
:
Grinding station : 25 kgs
Control box : 8 kgs
Protections of user :
Mask for aerosols
Protection glasses for possible liquid nitrogen spillage
The supply includes:
Grinding station, control box, DEWAR liquid nitrogen
reservoir with its sampling cane, connections and tubings
to DEWAR and nitrogen gas bottle.
CRYOPIX®
is a protected technology by many issued patents.
CRYOPIX®
is a registered trade mark.
All the images presented in this document have been
obtained with the courtesy of the Laboratoire de RadioAnalyse,
Cancer Research Center Val D'Aurelle, Montpellier, France.
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,
Ferme des Ebisoires, Impasse Paul Langevin, 78370 Plaisir - France
Tel +33 (0)1 30 07 52 95 - Fax +33 (0)1 30 07 51 56 - Email info@alphelys.com
Copyright © 2001/2007 ,
all rights reserved.
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