|
|
| Rapid Tissue Freezing |
| SnapFrost®, ultra-fast specimen freezing system |
|
| Reliable and fast frozen diagnosis procedure |
| |
|
An accurate intraoperative frozen diagnosis is important when deciding the surgical strategy against tumour. Frozen section diagnosis has been qualified by numerous studies as “fairly” reliable in approximately 90 % of the studied cases. The morphological quality of frozen section is critical to guarantee a high rate of reliability.
Most of frozen diagnosis is currently based on specimen freezing on cryostat freezing bar.
Although a freezing bar is at low temperature say -40°C or -50°C, it is not low enough to guarantee a fast and quality freezing of the specimen. Ice crystals can form and degrade the structures and walls of the specimen. Staining will not remain in the targeted compartments, structures will almost all look the same rendering the identification of the different morphologies more difficult and some times approximative. Images below are typically demonstrating such artefacts created by a low quality freezing procedure. The image on the right is produced out of a SnapFrost® frozen specimen. Staining is sharp and diagnosis far easier and reliable. |
 |
| |
|
  |
H&E section of human bladder.
Left: frozen on a freezing bar at -40°C in a cryostat, Right: frozen with SnapFrost® at -80°C. |
|
| Frozen specimens of reproducible controlled quality |
| |
|
 |
Cryostat freezing bar or liquid nitrogen deep freezing are not controlled freezing procedures and frozen specimen of variable quality can be produced.
SnapFrost® 2 is a temperature controlled deep freezer. You freeze your specimen in a controlled environment ensuring a reproducible high quality for frozen |
diagnosis.
As well as for fixation, deep freezing suffers from important variations of quality upon methods, procedures and operators habits.
SnapFrost® 2 controls temperature permanently and allows to strongly reducing quality variations and guarantees high quality tissue banks. You can safely rely on your frozen specimen for your most critical research work. |
|
| Safe snap freezing procedure |
| |
|
 |
Some times for frozen section diagnosis but also frequently used for tissue banking for research and diagnosis reserve, liquid nitrogen freezing remains a well used deep freezing procedure.
Although the method is fast due to ultra low temperature, conditions are not controlled.
Moreover, operations are not safe for the operator or even the lab. Regulations have been established defining the environment conditions for liquid nitrogen freezing. These regulations tend to make liquid nitrogen freezing more and more complex and less flexible when you need to have a dedicated room with safety alarms. Such kind of rooms cannot be established close to surgery theatre when you need to freeze as quickly as possible after resection to reduce RNA degradation.
SnapFrost® 2 is a compact deep freezer that can be |
|
moved anywhere you need it to do your freezing. Free of danger, it can be moved to the operation room for immediate freezing to generate frozen specimens of very high molecular content.
On use, SnapFrost® will prove also very economical, the coolant used, isopentane, being much cheaper that liquid nitrogen and a lot safer to handle and store! |
 |
|
|
| Flexible and convenient snap freezing instrument |
| |
SnapFrost® 2 allows you to freeze virtually any kind of tissue specimen.
It provides two chambers, one being set at -40°C and the other one including adjustable (option) temperature from -40°C down to -80°C or even -100°C (option).
The larger chamber at -40°C can be used to store frozen specimens until you have completed the freezing session to transfer the specimen to deep freezers.
Specimen can be dry frozen on cryostat holder for example or frozen in isopentane bath, more efficient and faster.
SnapFrost® 2 also includes a timer that allows you to program low temperature operation along the week so that the instrument is ready when you start freezing at time of need. |
| |
| |
| Literature |
| |
| Steu, S., M. Baucamp, G. von Dach, M. Bawohl, S. Dettwiler, M. Storz, H. Moch, and P. Schraml, A procedure for tissue freezing and processing applicable to both intra-operative frozen section diagnosis and tissue banking in surgical pathology. Virchows Arch 2008. |
|
| Download demonstration. |
|
|
,
Ferme des Ebisoires, Impasse Paul Langevin, 78370 Plaisir - France
Tel +33 (0)1 30 07 52 95 - Fax +33 (0)1 30 07 51 56 - Email info@alphelys.com
Copyright © 2001/2008 ,
all rights reserved.
|
|
|
