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| Molecular
Histology |
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| • FAQ • |
Tape transfer
technology CRYO-JANE®
for frozen tissue sections cutting out and mounting |
| Cryo-Jane® is an exclusive
and patented technique for the cutting out and mounting
of frozen tissue sections on microscope slide. |
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Mouse epiderma
stained
in HES |
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CRYO-JANE®
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| The
CRYO-JANE® technical principle |
The CRYO-JANE® technique lays on an
adhesive tape which allows the transfer of the tissue
section from the block out to the slide.
Operations of cutting out and transfer are simple and
easy : |
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1
The adhesive tape is sold in roller of 400 detachable
sections, ready for use. An adhesive tape section is detached
from the roller then stuck on the front face of the tissue
block frozen beforehand fixed on the cryotome. |
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2 The block
is cut out. The blade of the cryotome passing behind the
adhesive tape, the tissue section is found retained by
adhesion on the tape. The cut can now be easily transported
without risks nor damage. It remains frozen and can be
stored in the cryotome while waiting for the assembly
on the slide. Handling is extremely facilitated. |
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3
To transfer the tissue cut on the CRYO-JANE® slide,
the adhesive tape is applied, tissue cut in contact with
the slide, maintained in the cryotome. A roller, also
maintained with the cold, is applied to ensure a good
contact of the cut to the slide. The slide rests on a
support controlled in temperature which ensures the ideal
conditions of adhesion of the tissue cut on to the slide. |
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4 The slide
is placed under the UVs lamp, always in the cryotome and
is exposed to UVs (360 Nm) during a short moment of approx.
8 msec. The CRYO-JANE® slide has a polymer on its
surface which hardens under exposure to UVs and creates
strong bonds between the slide and the tissue section.
The lamp can function only cap dropped on the slide, thus
getting a high-level protection for the operator. |
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5
Once the polymer is hardened, the tissue cut is fixed
perfectly on the slide. The adhesive tape can be withdrawn.
The tissue section can then be dehydrated or dried with
the cryostat or be dried to air before fixing and staining.
The polymer is resistant to all types of solvents and
dyes and can be used in special or routine procedures,
in traditional or molecular histology. During all the
process, the tissue section remained perfectly frozen
for a better morphology, the stainings being more contrasted
for an easier diagnosis. |
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6 Once your
cut out work completed, protection oil maintained at a
temperature of 8°C in a thermostated bath installed
in the cryotome is applied to the front face of the block.
While solidifying itself, the oil forms a layer of protection
preventing the dehydration of the block during storage
at low temperature. Thus protected, your sample can be
stored without superabundant packing in your freezer for
long periods. During the next use, it is enough to surface
the block in order to update the block face again. |
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Please
Wait For Slide Show
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1. Snap-Freezing & Embedding
;
2. Cutting the Section Cryo-Vac-Away Nozzle Shown ;
3. Transfer to Slide ;
4. Curing the Adhesive Coating ; |
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5. Removal of Tape ;
6. Ready for Fixation & Staining Protocol ;
7. Mouse Liver H&E. |
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CRYO-JANE®
preserves tissue sections morphology
The tape, while adhering to the surface of the frozen
block, avoids the deformations which the passage of the
blade of the cryotome can involve. In addition, the tissue
cut being retained on the adhesive tape, this one is protected
from the deformations that can occur until assembly on
the CRYO-JANE® slide.
CRYO-JANE® preserves
integrity and localization of tissue sections components
for molecular biology
Each of the operations of the CRYO-JANE® technique
is carried out inside the cryotome. The accessories and
consumable, adhesive tape, microscope slide and roller,
are stored inside the cryotome. Thanks to these precautions,
the tissue cut is preserved frozen until its mounting
on the slide, the mounted slide being stored in the cryotome
until fixing, drying or another operation of treatment.
The CRYO-JANE® technique eliminates the risks from
defrosting and the formation of water which causes the
solubilization of certain molecules that can be partially
or totally degraded, displaced within the tissue cut,
distorting interpretations, or even their washing out
of the cut as of the first stage of treatment while staining.
The results are still improved by using the ultra-fast
freezing kit GENTLE-JANE®. ALPHELYS thus proposes
a whole of solutions to you to preserve your samples frozen
from sampling to molecular biology. |
CRYO-JANE®
eliminates tears and precious material losses
The adhesive tape CRYO-JANE® maintains the integrity
of the tissue at the time of its cut and its transfer
on the slide. It eliminates the risks of tears due to
a defect from the blade of cryotome, a fragile tissue,
etc, and which can make interpretations difficult. It
eliminates the repeated trials to obtain a good cut and
helps you to preserve your invaluable samples. The CRYO-JANE®
transfer tape enables you to save time while enabling
you to make a success of your cut each time.
CRYO-JANE® eliminates
folds and compressions, ideal for a reliable and sure
analysis and for laser microdissection LCM
The adhesive tape CRYO-JANE® adheres perfectly to
the frozen sample block, thus eliminating the risks from
folds which can be formed at the time of the cut or the
transfer of the tissue cut on the microscope slide. The
adhesive tape CRYO-JANE® thus makes it possible to
prepare perfectly plane and noncompressed, ideal tissue
cuts for work with laser microdissection on Arcturus Pixcell
LCM by guaranteeing to you a perfect contact between your
tissue cut and standard Capsure® of your microdissection
instrument. A plane cut is particularly recommended for
the microdissection of single cells or small structures
such as epitheliums. |
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CRYO-JANE®
allows ultra-thin sections down to 2 µm
The adhesive tape CRYO-JANE® reinforces sample while
adhering perfectly to its surface and allows you to prepare
finer cuts than usual, down to 2 µm. A finer cut
can bring to you advantages in faster staining procedure,
more precise analysis or exploitation of your cut with
other techniques such as FISH (In situ Fluorescence Hybridization)
where the smoothness of the cut can facilitate the penetration
of the genomic probes or the PCR In situ.
CRYO-JANE® provides
a strong attachment of the section to the slide, even
for fat tissues
Is easy to use, adapted for routine use
Adhesion of the tissue cuts varies in a very significant
way according to the tissue studied and constrained with
particular precautions as for the experimental conditions
of labeling of the slide, the type of slide, etc. The
CRYO-JANE® slides are covered with a polymeric which
hardens under exposure to UVs and gets an extremely strong
fastener of the tissue cut on the slide, the tissue being
strongly fatty or aqueous. The polymer, once hardened,
is resistant to the proteases, solvents alkaline and acids
and majority of organic solvents. The CRYO-JANE®
technique preserves your tissue cuts for a maximal use.
CRYO-JANE®
is easy to use, adapted for routine use
The CRYO-JANE® technique is available in form of
a kit ready for use including the accessories and a first
set of the consumables. The consumables can then be bought
separately. Consequently technique taken in hand, its
proves very simple and fast to use. The CRYO-JANE®
technique do not require any particular maintenance and
currently can fit on the majority of the existing cryostats.
Please specify the mark and the model of your cryostat
to the order. |
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Transfer Tape CryoJane® Kit for
frozen tissues. |
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| To order |
The kit is delivered complete
with :
- A roller of adhesive tape sections for 400 cuts ;
- 125 pre-treated slides ;
- A UVs lamp ;
- A roller.
Thank you to specify cryostat model upon order.
Catalog # 02-CJ. |
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Ferme des Ebisoires, Impasse Paul Langevin, 78370 Plaisir - France
Tel +33 (0)1 30 07 52 95 - Fax +33 (0)1 30 07 51 56 - Email info@alphelys.com
Copyright © 2001/2008 ,
all rights reserved.
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